Characterization of ouabain-resistant mutants of a canine kidney cell line, MDCK.
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Characterization of insulin-like growth factor I receptors on Madin-Darby canine kidney (MDCK) cell line.
Specific insulin-like growth factor I (IGF-I) receptors on the Madin-Darby canine kidney (MDCK) cell line were identified and characterized. [125I]IGF-I specifically bound to the cells, but [125I]insulin bindings to the cells was minimal. Unlabeled IGF-I displaced both the IGF-I and insulin bindings with potencies that were 100 and 10 times as great as insulin. By an affinity labeling technique...
متن کاملGrowth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.
Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with five factors--insulin, transferrin, prostaglandin E1, hydrocortisone, and triiodothyronine--as a serum substitute. These five factors permit growth for 1 month in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl adenosine 3',5'-cyclic monophosphate subs...
متن کامل(CFTR)-mediated chloride secretion in type I Madin- Darby canine kidney (MDCK) cell line
word count: 248 11 Corresponding Author: 12 Sunhapas Soodvilai, Ph.D. 13 Department of Physiology 14 Faculty of Science, Mahidol University 15 Rama VI Road, Bangkok 10400 Thailand 16 Phone number: +66-2-2015516 17 Fax number: +66-2-3547154 18 Email:[email protected] 19 Articles in PresS. Am J Physiol Renal Physiol (May 29, 2013). doi:10.1152/ajprenal.00579.2012 Copyright © 2013 by the ...
متن کاملGangliosides stimulate dome formation in cultured canine kidney epithelial cell line (MDCK).
The effect of exogenous gangliosides on the occurrence of domes in MDCK cell cultures was investigated in view of the involvement of both dome formation and gangliosides in cell growth, differentiation and transepithelial transport. Dome formation was increased by gangliosides in medium free of fetal calf serum. Among the gangliosides tested, GM3 and GD3 isolated from porcine kidney were most a...
متن کاملMadin-Darby Canine Kidney Epithelial Cell Line
The Madin-Darby canine kidney (MDCK) cell line, derived from distal tubule/ collecting duct, expresses differentiated properties of renal tubule epithelium in culture. We studied the expression of adrenergic receptors in MDCK to examine the role of catecholamines in the regulation of renal function. Radioligand-binding studies demonstrated, on the basis of receptor affinities of subtype-selecti...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)44174-3